What is PCR? Polymerase chain reaction (PCR) is a common laboratory technique used to make many copies (millions or billions!) of a particular region of DNA. This DNA region can be anything the …

PCR (Polymerase Chain Reaction) is a vital technique in molecular biology, enabling researchers to amplify specific DNA fragments exponentially. Essential for cloning, forensics, and medical …

For your first question: You will do PCR of the entire sample. Then a restriction enzyme is used to cut the part you want. One restriction enzyme will make the cut on the same place in all samples. So you …

Since the DNA samples from vial 3 have three different lengths, does that mean three different target sequences were replicated when PCR was done and all three were later put into vial 3?

Figure 1 Schematic of RT-PCR experiment. The position of the reverse transcriptase primer (Primer 1) and the positions of the PCR primers (Primers A and B) are shown. They then analyzed their …

ATP structure, ATP hydrolysis to ADP, and reaction coupling.

In the diagram below, mRNAs are being transcribed from several different genes. Although transcription is still in progress, ribosomes have attached each mRNA and begun to translate it into protein.

In the polymerization reaction mentioned in the section,"DNA Polymerase", shouldn't the diagram be showing a nucleoSIDE triphosphate instead of a nucleotide triphosphate?

That’s because, during a ligation, DNA fragments don’t always get “pasted” in exactly the way we intend. Instead, we must collect DNA from several colonies and see whether each one contain the right …

Although genomes are now typically sequenced using other methods that are faster and less expensive, Sanger sequencing is still in wide use for the sequencing of individual pieces of DNA, such as …