Gene targeting, the use of homologous recombination in mouse embryonic stem (ES) cells to modify mouse genes precisely 1,2,3,4,5, allows researchers to create virtually any desired modification in the ...

Polymerase chain reaction (PCR) test kits for COVID-19 use what’s known as gene cloning – not reproductive cloning – to detect the presence or absence of the SARS-CoV-2 virus, but social media posts ...

Positional cloning is a genetic mapping technique used to pinpoint the location of specific traits of interest, such as disease-causing genes or mutations, within the genome. Very simply, this ...

Mammalian cell-based production systems are gaining increasing importance in the manufacture of biotherapeutics. The major advantage of mammalian cell expression platforms over bacteria and yeast is ...

Here are steps you can take to increase the odds of generating correct clones. PCR amplicons are commonly used for cloning. Primers made from synthetic oligonucleotides often contain a small ...

Organelle genomics has unearthed an unsuspected wealth of genetic diversity of mitochondrial DNAs (mtDNAs) and plastid (chloroplast) DNAs across eukaryotes. In addition, these genomes display ...