Cell or tissue lysates are arrayed on nitrocellulose-coated slides (Grace Bio-labs, Bend, OR) in triplicate using the Aushon 2470 Arrayer (Aushon BioSystems, Billerica, MA) (technical replicates).
Image manipulation scandals and tightening journal guidelines have put traditional Western blots under a spotlight: every manual step—from gel casting to image ...
The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins has been in practice for four decades. In recent ...
As gene therapies continue to advance, the demand for scalable, reproducible, and efficient potency assays is rapidly increasing—especially for adeno-associated virus (AAV) vectors. Accurate ...
The field of targeted protein degradation (TPD) is advancing at an unprecedented pace, offering novel therapeutic strategies for addressing previously undruggable targets. However, efficient and ...